. Author manuscript; available in PMC: 2010 Mar 18.

Published in final edited form as: Biochemistry. 2009 Jun 16;48(23):5180–5186. doi: 10.1021/bi9000672

Table 1.

Membrane-Binding Experiments^a

construct	added calcium	K_p(μM)
full length	no	77 ± 36
full length	yes (500 nM)	85 ± 18
N-terminus, His tagged	no	188 ± 41
N-terminus, untagged	no	187 ± 38

Lipids were in the form of large unilamellar vesicles (0.1 μm) composed of POPC:POPS:POPE (1:1:1). The buffer was 20 mM HEPES, pH 7.4, and 0.16M NaCl.