Figure 6.

Levels of iNOS message in the intestine and liver of C57BL/6 mice infected with T. gondii. Mice were infected orally with cysts. On day 0, 4, and 7 p.i. the liver and distal ileum were isolated and stored at −70°C. The pooled tissues from three mice at each time point were homogenized, and mRNA expression for iNOS was assayed by reverse transcription–PCR. The transcriptional level is expressed relative to uninfected mice (designated as 1). The cDNA concentration examined at each point was standardized to the hypoxanthine phosphoribosyltransferase mRNA level (not shown).