Table 3.
Comparison of K. brevis culture recovery after handpicking or electronic single-cell sorting
Single-cell isolation approach for CCMP2229 | Electronic sorting | Handpicking |
---|---|---|
No. of single-cell isolation attempts | 192 | 192 |
Isolations surviving 2 generations (4 cells) | 39 | 43 |
Isolations surviving 5 generations (32 cells) | 6 | 4 |
Isolations surviving 10 generations (1024 cells) | 6 | 2 |
Isolations surviving minimum of 3 months and generating large scale (> 4 1) cultures | 4 | 1 |
% Successful isolation recovery | ~ 2% | ~ 0.5% |