Figure 5.

hh pathway genes are upregulated in ependymal cells of the lesioned spinal cord. Cross-sections of the adult spinal cord at the level of the central canal (asterisk) are shown. A, Expression of patched1 and smoothened mRNA is increased after a lesion. Note polarized (stronger ventral) expression of patched1 mRNA, whereas smoothened mRNA is detectable along the dorsoventral extent of the central canal of the lesioned spinal cord. B, In the lesioned, but not the unlesioned spinal cord, Tg(olig2:egfp)⁺ ependymoradial glial cells express detectable levels of mRNA for patched1 (arrow), an indicator of hh pathway activity. Scale bars: A, 25 μm; B, 15 μm.