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Na+ and Li+ compete with K+ ions for the Vc-NhaP2 antiporter. Dequenching of acridine orange in response to varying concentrations of KCl was monitored at pH 7.5 in standard choline chloride buffer with or without the indicated concentrations of NaCl or LiCl. Each point represents the average of four measurements (carried out in duplicate with two separate isolations of vesicles). Data are shown in reciprocal coordinates. Bars show the standard deviation.
