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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Protein Expr Purif. 2009 Dec 6;70(2):196–205. doi: 10.1016/j.pep.2009.12.003

Fig. 1.

Fig. 1

Experimental pI of T7-tagged fragment of CBP spanning the CH3 domain and partial Q-domain, CBP CH3. (A) Diagram of different domains of CBP, including partial list of known interacting proteins at the C-terminus. Location of the Akt consensus site within the cloned fragment of mouse CBP is indicated. The analogous Akt consensus sites at human CBP and p300 are also shown. (B) Two-dimensional gel electrophoresis of CBP CH3 lysates and subsequent detection of resolved proteins via Coomassie blue staining and (C) parallel Western blotting against T7 tag and (D) Akt substrate motif.