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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Protein Expr Purif. 2009 Dec 6;70(2):196–205. doi: 10.1016/j.pep.2009.12.003

Fig. 3.

Fig. 3

Elution profiles of clarified lysates of CBP CH3 via (A) hydroxyapatite chromatography and subsequent (B) size-exclusion chromatography of pooled fractions from previous purification step. Indicated in circles are peaks corresponding to T7-tagged CBP CH3 proteins as assessed by Western blotting (see Materials and methods and Fig. 4).