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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Protein Expr Purif. 2009 Dec 6;70(2):196–205. doi: 10.1016/j.pep.2009.12.003

Fig. 8.

Fig. 8

In vitro phosphorylation assays of CBP CH3 fragments and GSK-3α/β fusion protein in the absence and presence of Akt1. 32P-labeled proteins were identified in PVDF membrane by autoradiography. Arrow indicates the location of 32P-CBP CH3 fragments, while star indicates the location of 32P-GSK-3α/β.