. Author manuscript; available in PMC: 2011 Apr 1.

Published in final edited form as: Protein Expr Purif. 2009 Dec 6;70(2):196–205. doi: 10.1016/j.pep.2009.12.003

Table 2.

Protein recovery from GST pull-down assays

Eluted Proteins from GSH Sepharose	Percentage of recovery^b
WT CBP CH3	1.67 ± 0.67
GSK-3α/β	31.7 ± 13.5
Akt1	34.4 ± 2.0

Densitometric scanning of observed bands in stained gels relative to band density of starting amount of each protein used in the assays.