Table 1.

Overview of sporozoite proteins characterized through reverse genetics techniques

Gene	Locationa	Targeting signals	Domains	Gene knockout phenotype	Likely function
AMA1	Micronemes	SP/TM	Hypotheticalf	Refractory to deletion	Erythrocyte invasion and possibly hepatocyte invasion
CelTOS/S4	Micronemes	SP	Hypothetical	Defect in ookinete midgut traversal/sporozoite transmission to the liver	Ookinete/sporozoite cell traversal
CSP	Surface	SP/GPI	TSRd	Lack of sporozoite formation	Multiple essential functions throughout preerythrocytic stage development
P52/P36p	Micronemes	SP/GPI	6-Cysteine	Defect in early LS development	Hepatocyte invasion/PVM formation?
P36	Secretory organelles	SP	6-Cysteine	Defect in LS development	Hepatocyte invasion/PVM formation?
PL/UIS10	Secretory organelles	SP	LCAT	Partial defect in sporozoite transmission to the liver	Sporozoite cell traversal
SAP1	Cytoplasm	None	Hypothetical	Blockage of LS development	Regulation of liver-infectivity-associated gene expression
SPECT1	Micronemes	SP	Hypothetical	Defect in Kupffer cell traversal/skin traversal	Sporozoite cell traversal
SPECT2/PLP1	Micronemes	SP/TM	MACPF	Defect in Kupffer cell traversal/skin traversal	Sporozoite cell traversal
TLP	Secretory organelles	SP/TM	TSR and A-domain	Partial defect in sporozoite transmission to the liver	Sporozoite cell traversal
TRAP	Micronemes	SP/TM	TSR and A-domaine	Blockage of salivary gland invasion	Sporozoite motility, salivary gland invasion, and hepatocyte invasion
TRSP	Secretory organelles	SP/TM	TSR	Partial defect in hepatocyte invasion	Hepatocyte invasion
UIS3	Secretory organelles/PVM	SP/TM	Hypothetical	Arrest of LS development	Unidentified essential function in LS growth/likely fatty acid uptake
UIS4	Secretory organelles/PVM	SP/TM	Hypothetical	Arrest of LS development	Unidentified essential function in LS growth
UOS3/S6/TREP	Micronemes	SP/TM	TSR	Blockage of salivary gland invasion	Sporozoite motility/salivary gland invasion

^aPutative subcellular localization based on available data

^d(in the abbreviations list)

^e(in the abbreviations list)

^fNo known functional domain identified