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. Author manuscript; available in PMC: 2010 Sep 4.
Published in final edited form as: Nature. 2010 Mar 4;464(7285):121–125. doi: 10.1038/nature08778

Figure 1. Fasting induces SIRT3 expression in oxidative tissues.

Figure 1

a. Mitochondria were isolated from livers of fed or fasted (6-48 h) wt mice and analyzed for SIRT3 expression by western blot analysis, electron transfer flavoprotein (ETF) was used as a reference; b. Mitochondria isolated from livers of fed or fasted wt and SIRT3-/- mice were analyzed by western blotting analysis with an antiserum specific for anti-acetyllysine, ATP synthase alpha was used as a reference. The arrows identify candidate SIRT3 target proteins: deacetylated during fasting in wt mice, relatively hyperacetylated and not deacetylated during fasting in SIRT3-/- mice.