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. Author manuscript; available in PMC: 2010 Sep 4.
Published in final edited form as: Nature. 2010 Mar 4;464(7285):121–125. doi: 10.1038/nature08778

Figure 3. Defective fatty acid oxidation in mice lacking SIRT3-/-.

Figure 3

a, b. Fatty acid oxidation was measured by incubation of liver extract from wt and SIRT3-/- mice with 14C-palmitate, acid-soluble metabolites [(ASM), panel a] and captured CO2 (panel b), n=10/genotype; c. Mitochondrial fatty acid oxidation was measured in other oxidizing tissues, including heart, liver, mixed gastrocnemius and soleus skeletal muscle (SKM), and brown adipose tissue (BAT) (CO2, n=7/genotype, 100 μM substrate), d. Fatty acid oxidation was measured by incubation of 14C-palmitate (100 μM) in liver extract from wt and SIRT3-/- mice one week after injection with adenovirus expression vectors for GFP or SIRT3 (ASM, n=3-4/category); *p<0.05, **p<0.01.