Table 1.
Comparison of the effects of immunosuppressants and H2O2 on the concentration of 13C-labeled glucose, lactate and glutamate in perfused rat brain slices, C6 glioma cells and primary astrocytes as calculated from 13C-NMR spectra of water soluble PCA-extracts.
| Control | CsA | SRL | RAD | CsA+SRL | CsA+RAD | Positive Control (H2O2) |
||
|---|---|---|---|---|---|---|---|---|
| C3-Lactate | Slices | 1602.5±169.2 | 2042.5±355.4* | 1122.3±103.4* | 1025.0±141.5** | 927.4±54.4** | 1455.0±174.6 | 847.5±227.1** |
| Astrocytes | 855.0±95.7 | 1020.0±69.8* | 707.5±66.5* | 732.5±68.0 | 667.5±124.2* | 1005.0±270.9 | 600±128.8* | |
| C6 | 185.5±11.9 | 120.1±10.4* | 67.3±21.0** | 75.6±11.0*** | 103.3±11.4*** | 85.5±13.7*** | 34.5±13.2*** | |
| C4-Glutamate | Slices | 562.0±149.5 | 338.0±57.2* | 612.4±145.1 | 482.3±130.6 | 337.6±47.2* | 464.7±129.3 | 324.9±89.3* |
| Astrocytes | 701.7±39.0 | 523.4±85.5* | 690.1±83.8 | 692.8±70.3 | 519.9±75.6* | 825.2±356.4 | 587.4±162.0 | |
| C6 | 1290.9±60.4 | 1000.9±264.9 | 730.1±112.0** | 675.7±207.9* | 752.4±189.0* | 815.8±257.1** | 246.9±129.9*** | |
| Glucose | Slices | 3164.6±315.5 | 3395.6±465.8 | 3579.9±359.1 | 2598.1±538.3 | 2640.2±226.8* | 2734.2±591.1 | 1343.7±366.6*** |
| Astrocytes | 2284.7±138.9 | 2037.6±152.1* | 2133.1±207.7 | 2205.2±330.6 | 2551.5±327.1 | 2391.0±1101.3 | 1662.3±262.9* | |
| C6 | 3326.7±482.1 | 3493.0±775.5 | 5059.6±939.8* | 4082.4±1146.1 | 4589.7±1300.5* | 3738.7±782.8 | 2904.1±740.1 | |
Results are presented as means ± standard deviations (n=4, *p<0.05, **p<0.005, ***p<0.001) in nmol/g cell weight. Rat brain slices, C6 glioma cells and primary astrocytes were treated with CsA (500 µg/L), SRL (100 µg/L), RAD (100 µg/L), alone or in combination for 3 hours or with H2O2 (0.1 mM for cells and 1 mM for brain slices) for 30 minutes. During the drug treatment 5mM [1-13C]-glucose was added into the cell/brain slices incubation medium for 3 hours. Bold: rat brain slices italic: primary astrocytes, normal: C6 glioma cells.