Fig. 2.
SesB knockdowns increase basal calcium levels and blocks neuropeptide stimulation. A, B: expression of sesB RNA interference (RNAi) was targeted to principal cells in adult tubules expressing either the cytosolic (A) or mitochondrial (B) targeted calcium reporter, apoaequorin. Tubules were stimulated with the neuropeptide Capa-1 (10−7 M, arrowed), and the typical calcium response shown in black. Data are expressed as mean [Ca2+] (nM) ± SE; n > 10, P < 0.05, compared with wild-type tubules. C: bar graphs represent cytosolic and mitochondrial basal [Ca2+] from tubule principal cells overexpressing targeted sesB RNAi, average [Ca2+] (nM); n = 6, where ***P < 0.0001, Student's t-test. D: expression of sesB RNAi was targeted to stellate cells in adult tubules expressing the cytosolic calcium reporter. Tubules were stimulated with the neuropeptide Drosophila leucokinin (Drosokinin, 10−7 M, arrowed). Overexpression of sesB RNAi resulted in a significantly decreased Drosokinin calcium response.