Table 4.
Thymus, spleen, mesenteric lymph nodes, and peripheral blood immune cell phenotype of DR.lepr−/lepr− line
| DR.+/+ | DR.lepr−lepr− | P | |
|---|---|---|---|
| Thymus | |||
| DN | 5.35 ± 1.1 | 8.06 ± 2.8 | ns |
| DP | 82.04 ± 0.8 | 78.18 ± 2.4 | * |
| CD4+ | 7.89 ± 0.7 | 10.03 ± 0.5 | * |
| CD8+ | 4.73 ± 0.6 | 3.73 ± 0.3 | * |
| R73+ | 94.55 ± 0.9 | 89.50 ± 2.6 | * |
| CD4+CD25+ | 0.89 ± 0.2 | 0.79 ± 0.1 | ns |
| CD90+ | 97.31 ± 1.1 | 93.25 ± 2.8 | * |
| CD45+CD45RA+ | 0.63 ± 0.2 | 0.49 ± 0.1 | ns |
| CD45+CD45RC+ | 0.72 ± 0.1 | 0.87 ± 0.2 | ns |
| CD45+ | 97.08 ± 1.1 | 93.99 ± 3.4 | ns |
| Spleen | |||
| R73+ | 25.08 ± 1.5 | 20.25 ± 1.7 | * |
| R73+CD4+ | 63.41 ± 1.7 | 67.99 ± 1.4 | * |
| R73+CD8+ | 27.59 ± 1.1 | 21.03 ± 1.2 | * |
| R73+CD4+CD8+ | 4.18 ± 0.3 | 5.98 ± 0.6 | * |
| R73+CD4−CD8− | 3.98 ± 0.4 | 2.45 ± 0.2 | * |
| CD4+CD25+ | 3.14 ± 0.3 | 3.21 ± 0.4 | ns |
| CD90+ | 29.55 ± 2.4 | 24.93 ± 1.5 | * |
| CD45+CD45RA+ | 42.23 ± 2.5 | 32.32 ± 1.1 | * |
| CD45+CD45RC+ | 30.85 ± 2.6 | 19.15 ± 1.1 | * |
| CD45+ | 92.25 ± 1.6 | 84.86 ± 2.7 | * |
| MLN | |||
| R73+ | 75.30 ± 3.9 | 77.20 ± 7.5 | ns |
| R73+CD4+ | 77.98 ± 6.3 | 76.07 ± 3.0 | ns |
| R73+CD8+ | 14.66 ± 4.5 | 14.39 ± 3.7 | ns |
| R73+CD4+CD8+ | 2.26 ± 0.4 | 3.39 ± 0.8 | * |
| R73+CD4−CD8− | 1.71 ± 0.2 | 1.66 ± 0.6 | ns |
| CD4+CD25+ | 6.03 ± 1.1 | 6.66 ± 0.7 | ns |
| CD45+CD45RA+ | 18.29 ± 3.5 | 17.31 ± 2.5 | ns |
| CD45+CD45RC+ | 20.60 ± 3.3 | 19.91 ± 2.4 | ns |
| CD45+ | 98.05 ± 1.6 | 96.93 ± 2.5 | ns |
| Peripheral blood | |||
| R73+ | 31.08 ± 4.8 | 19.83 ± 6.7 | ns |
| R73+CD4+ | 80.25 ± 2.3 | 78.52 ± 1.9 | ns |
| R73+CD8+ | 16.31 ± 3.2 | 14.60 ± 3.9 | ns |
| R73+CD4+CD8+ | 1.75 ± 0.3 | 3.09 ± 0.4 | * |
| R73+CD4−CD8− | 0.93 ± 0.4 | 0.69 ± 0.0 | ns |
| CD4+CD25+ | 0.95 ± 0.3 | 1.20 ± 0.1 | ns |
| CD45+CD45RA+ | 12.93 ± 1.4 | 13.13 ± 4.3 | ns |
| CD45+CD45RC+ | 19.25 ± 2.7 | 19.10 ± 5.1 | ns |
| CD45+ | 33.83 ± 7.1 | 38.40 ± 7.1 | ns |
Data are from male and female rats and represent means ± SD for n = 4/group for each genotype. Values are the proportion (%) of the total gated cells as determined by immunofluorescence. MLN, mesenteric lymph node. Differences among DR.+/+ control and DR.lepr−/lepr− rats were determined by Student's t-test.
*
P < 0.05.