Table 3.
Cells infected with the cp-52 mutant virus do not bind murine mAbs directed at epitopes shared by subgroup A and subgroup B RSV G glycoproteins
| mAb | Protein specificity of mAb | Subgroup specificity of mAb | Reactivity of indicated RSV with Ab
|
||
|---|---|---|---|---|---|
| A2 wt | B1 wt | cp52 | |||
| 131-2g | G | A,B | + | + | − |
| 130-5f | G | A,B | + | + | − |
| L9 | G | A,B | + | + | − |
| 92-11C | F | A | + | − | − |
| 102-10B | F | B | − | + | + |
Plaque immunostaining was performed as previously described (14). Vero cell monolayers in 24-well plates were inoculated with 50 pfu of indicated virus, incubated for 5 days at 37°C, fixed with methanol, and stained with RSV F- or G-specific mAbs. Each G-specific mAb was used at a dilution of 1:200. Each F-specific mAb was used at a dilution of 1:1,000.