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. Author manuscript; available in PMC: 2010 Mar 18.
Published in final edited form as: Biochemistry. 2008 Sep 20;47(41):10871–10880. doi: 10.1021/bi800930e

Figure 4.

Figure 4

Global tyrosine phosphorylation analysis of Src and mutants. SYF cells were transiently transfected with c-Src or mutants. Cells were harvested 40 h post-transfection, and lysates (50 μg) were separated via 10% SDS–PAGE and transferred onto a PVDF membrane. The membrane was probed with anti-pTyr antibody and reprobed with anti-Flag antibody to check expression. The membrane was probed with anti-tubulin antibody as a loading control. Vector represents SYF cells transfected with empty vector alone. The figure is representative of three separate experiments.