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. Author manuscript; available in PMC: 2010 Mar 18.
Published in final edited form as: Biochemistry. 2008 Sep 20;47(41):10871–10880. doi: 10.1021/bi800930e

Figure 7.

Figure 7

Phosphorylation of Sam68 by Src and the mutants. SYF cells were transiently transfected with wild-type Src or the mutants and arrested in mitosis using nocodazole 24 h after transfection. The mitotic cells were harvested 40 h post-transfection, and lysates were subjected to immunoprecipitation using anti-Sam68 antibody. The immunoprecipitates were separated by 10% SDS–PAGE, and Western blotting was carried out with anti-pTyr antibody. The membranes were stripped and reprobed with anti-Sam68 antibody to ensure equivalent Sam68 immunoprecipitation. The figure is a representative of three separate experiments; a comparison of results from three different transfected cell lysates is shown in Figure 4 of the Supporting Information.