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Affinity purification. Goat (a) or rabbit (b) serum was purified on an affinity column composed of streptavidin–agarose and biotinylated Fn3 (gI2.5.3T88I or rI4.5.5K27S/K56S). The serum (S), flowthrough (F), washes (W1–W3) and elution (E) were separated by polyacrylamide gel electrophoresis and stained with SimplyBlue SafeStain. L indicates the protein ladder.
