Table II.
Binder sequences
| Goat IgG binders |
Rabbit IgG binders |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Clone | # | BC loop | DE loop | FG loop | Framework | Clone | # | BC loop | DE loop | FG loop | Framework |
| WT | — | DAPAVTVR | GSKST | GRGDSPASSK | — | WT | — | DAPAVTVR | GSKST | GRGDSPASSK | — |
| Round 1 | |||||||||||
| gI1.5.1 | 1 | ALPRSE | GIRS | AHKSVL | S2P, T58I | rI1.5.1 | 1 | VRPSYSRL | STATT | GYGGRRVQ | P51A, G61R, Y73C |
| gI1.5.2 | 1 | HSYYSYY | GFYST | MDGASPLQ | D7G | rI1.5.2 | 1 | ATTGKAPL | KGATA | SYYDYHS | Q46K, S60R |
| gI1.5.3 | 2 | KMRAAR | RFRS | GDGHGG | T58I | rI1.5.3 | 4 | VATSCL | ATWVK | HYDDTLS | — |
| gI1.5.4 | 1 | NLEIFPR | GIRS | RTRVI | P51S, T58I | rI1.5.4 | 2 | ATTGKTPL | RSAEM | HYDDTLS | — |
| gI1.5.5 | 1 | NLGIFPR | GIRS | RTRVI | T58I | rI1.5.8 | 1 | VATSCL | ATWVK | HYDDTLS | N42G |
| rI1.5.9 | 2 | ARASNPCL | ATWVK | GYGGKRVQ | Q46R, S60R, K98E | ||||||
| Round 2 | |||||||||||
| gI2.5.1 | 1 | TNLSSS | NWTS | SYGLVISN | T58I, V68A | rI2.5.1 | 1 | VRSPYRRL | RSARS | GYGGRRVQ | — |
| gI2.5.2 | 1 | ALPRSE | NWTS | SPGLVLGA | T58I | rI2.5.2 | 2 | VNGDSCL | ATWVK | GYGGKRVQ | S60R |
| gI2.5.3 | 2 | TRAYFAP | GSLSS | SYGLVITD | P51S, T58I, I88T | rI2.5.3 | 1 | VRPSYSRL | PTHFF | GYGGKRVQ | S60R |
| gI2.5.4 | 2 | YSSYSYY | GFRPT | YYSSSYY | T35A, E38G, S89P | rI2.5.4 | 1 | ARPSYSRL | ATWVK | GYGGKRVQ | — |
| gI2.5.5 | 1 | RMPVTD | Truncation | — | rI2.5.5 | 2 | VRPSYSRL | ATWVK | GYGGKRVQ | — | |
| gI2.5.8 | 1 | RLPRSA | NWTS | SPGLILGA | T58I, I90T | rI2.5.6 | 1 | VRPSYSRL | KGATV | GYGGKRVQ | P51S |
| gI2.5.9 | 1 | YCSYSYY | GFRSG | FDGVAF | — | rI2.5.9 | 1 | VRPSYSRL | ATWVK | GYGGERVQ | — |
| rI2.5.10 | 1 | VATSCL | RSATS | GYGGKRVQ | P51S | ||||||
| Round 3 | |||||||||||
| gI3.2.1 | 2 | TARMRSP | NWTS | SPGLILGA | T58I | rI3.6.2 | 1 | VRPSYSRL | RSWTS | GYGGKRVP | S60R |
| gI3.2.2 | 2 | ALPRSE | NWTS | SPGLVLGA | T58I | rI3.6.3 | 2 | SRARNACL | ATWVK | GYGGKRVQ | G61R |
| rI3.6.4 | 2 | VRPSYSRL | RSARS | GYGGERVQ | P51S, S60R | ||||||
| gI3.5.1 | 5 | TRAYFAP | GSLSS | SYGLVITD | P51S, T58I, I88T | rI3.6.5 | 1 | AHAPNPCL | ATWVK | GYGGKRVQ | S60R, I90T |
| rI3.6.6 | 2 | ATTGKAPL | KGATA | SYYDYHS | Q46K, S60R | ||||||
| rI3.6.10 | 1 | ARPSYSRL | GSAHV | GYGGKRVQ | P51S, S60R, G61R | ||||||
| Round 4 | |||||||||||
| gI4.5.1 | 5 | TRAYFAP | GSLSS | SYGLVITD | P51S, T58I, I88T | rI4.3.1 | 2 | ATTGKTPL | RSAEM | HYDDTLS | — |
| rI4.3.3 | 1 | ARASNPCL | ATWVK | GYGGKRVQ | S60R | ||||||
| rI4.3.4 | 1 | VNGDSCL | GSAHV | GYGGKRVQ | P51S, S60R, G61R, A74T | ||||||
| rI4.3.5 | 1 | AHAPNPCL | AAWVE | GYGGKRVQ | S60R | ||||||
| rI4.5.1 | 4 | ATTGKAPL | ATWVK | HYDDTLS | S60R, K98E | ||||||
| rI4.5.5 | 1 | ATTGKAPL | ATWVK | HYDDTLS | S60R | ||||||
Clones are named as (g/r)Ix.y.z where g or r indicates goat or rabbit species specificity, x is the number of mutagenesis steps, y the number of selections after the most recent mutagenesis and z the clone number in that population. # indicates the frequency of occurrence of the indicated clone.