| Laboratory | aCSF Composition in mM |
| Pfaff, O’Donovan, Landmesser (3) | 128 NaCl, 4 KCl, 1.5 CaCl2, 1 MgSO4, 0.5 NaH2PO4, 21 NaHCO3, 30 glucose. |
| Goulding, Keihn (5) | 111 NaCl, 3.08 KCl, 2.52 CaCl2, 1.25 MgSO4, 1.18 KH2PO4, 25 NaHCO3, 11 glucose |
| Brownstone (6) | 127 NaCl, 1.9-3.9 KCl, 1.2 KH2PO4, 2.4 CaCl2, 1.3 MgCl2, 26 NaHCO3,10 glucose |
| Ziskind-Conhaim Dissection (7) | 113 NaCl, 3 KCl, 1 CaCl2, 6 MgCl2, 25 NaHCO3, 1 NaH2PO4, 11 glucose |
| Ziskind-Conhaim Extracellular (7) | 113 NaCl, 3 KCl, 2 CaCl2, 1 MgCl2, 25 NaHCO3, 1 NaH2PO4, 11 glucose. |
| O’Donovan, chick (8,9) | 139 NaCl, 2.9-5 KCl, 17 NaHCO3, 1 MgCl2, 3 CaCl2, 12 glucose |
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