Table 7.
Primer sequences for real time PCR.
| Gene | Forward Primer | Reverse Primer | Size | Accession no. |
|---|---|---|---|---|
| 5'-3' | 5'-3' | (bp) | ||
| ADAM12 | tttgggggtcaacagttttc | agagctgggttcccttttgt | 191 | NM_003474 |
| AREG | tggggaaaagtccatgaaaa | tttcgttcctcagcttctcc | 174 | NM_001657 |
| AGN2 | ccacctgaggaactgtctcg | ggtcttgctttggtccgtta | 191 | NM_001147 |
| ER | catatgggagaagggggagt | aagtgcaattacagagtgcaaaa | 166 | NM_001432 |
| JAGL1 | gcctgccttaagtgaggaaa | gccaagaacaacacatcaaaga | 169 | U77914 |
| LAMA5 | gtgatgaaaagcgggaatgt | acctccacagagcgagtcat | 221 | BC003355 |
| NOV | tgcaattccaagaaaatatcactg | cttggatttggagcttggaa | 167 | NM_002514 |
| PDGF-A | acacgagcagtgtcaagtgc | tctggttggctgctttaggt | 250 | X03795 |
| PN | cactctttgctcccaccaat | tcaaagactgctcctcccata | 157 | AY140646 |
| RL | tgctgaatttggggctactt | gggagatagggtcttcatcca | 198 | NM_005045 |
| SCG2 | cccgaagaatgatgataccc | aaatgttgggatttgcttgg | 195 | NM_003469 |
| ITGα5 | agttgcatttccgagtctgg | ccaaacaggatggctaggat | 223 | NM_002205 |
| β-actin | cacactgtgcccatctacga | ctccttaatgtcacgcacga | 162 | X00351 |
| gapdh | ctcctcctgttcgacagtca | gttaaaagcagccctggtga | 140 | NM_002046 |
Note: ADAM12, a disintegrin and matrix metalloproteinase 12; AREG, amphiregulin; AGN2, angiopoietin 2; ER, epiregulin; JAGL1, jagged soluble form; LAMA5, laminin alpha 5; NOV, nephroblastoma over expressed; PDGF-A, platelet-derived growth factor alpha; PN, periostin; RL, reelin; SCG2, secretogranin 2; ITGα5, integrin alpha 5; β-actin, beta-actin; gapdh, glyceraldehyde 3-phosphate dehydrogenase