Figure 2.

Western blot analysis of Fusion 1 (CotB-UreA1) performed with anti-CotB (A) or anti-UreA (B) of spore coat proteins extracted from strains PY79 (lanes 1) and KH17 (PY79 carrying cotB::ureA1) (lanes 2). In both panels arrows point to fusion proteins. Purified UreA was run in lane 3 of panels B. Twenty five micrograms of total proteins were separated on either 8% (A), 12% (B) polyacrylamide gels, electrotransferred to nitrocellulose membranes and reacted with primary antibodies and then with peroxidase-conjugated secondary antibodies and visualized by the enhanced chemiluminescence method.