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. 2010 Jan 18;9:2. doi: 10.1186/1475-2859-9-2

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Copyright ©2010 Hinc et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western blot analysis of Fusion 2 (CotC-UreA1) performed with anti-CotC (A) or anti-UreA (B) of spore coat proteins extracted from strains PY79 (lanes 1), KH10 (PY79 carrying cotC::ureA1) (lanes 2), RH101 (PY79 cotC::spc) (lanes 3), KH11 (PY79 cotC::spc cotC::ureA1) (lanes 4), RH209 (PY79 cotC::spc cotU::erm) (lanes 5) and KH12 (PY79 cotC::spc cotU::erm cotC::ureA1) (lanes 6). In both panels arrows point to fusion proteins. Purified UreA was run in lane 7 of panels B. Twenty five micrograms of total proteins were separated on either 15% (A) or 12% (B) polyacrylamide gels, electrotransferred to nitrocellulose membranes and reacted with primary antibodies and then with peroxidase-conjugated secondary antibodies and visualized by the enhanced chemiluminescence method.