Figure 4.

Western blot analysis of Fusion 3 (CotG-UreA) performed with anti-CotG (A) or anti-UreA (B) of spore coat proteins extracted from strains PY79 (lanes 1), ER203 (PY79 cotG::erm) (lanes 2) and KH23 (PY79 cotG::erm cotG::ureA) (lanes 3). In both panels arrows point to fusion proteins. Purified UreA was run in lane 4 of panel F. Twenty five micrograms of total proteins were separated on either 15% (A) or 12% (B) polyacrylamide gels, electrotransferred to nitrocellulose membranes and reacted with primary antibodies and then with peroxidase-conjugated secondary antibodies and visualized by the enhanced chemiluminescence method.