MGAS2221 |
Pharyngitis clinical isolate, serotype M1 |
Wild-type for covRS
|
[26]
|
2221ΔccpA
|
ΔccpA strain |
MGAS2221 ccpA::spc
|
This study |
comp2221ΔccpA
|
ΔccpA strain with CcpA complementation vector |
ΔccpA/ccpA+, Spcr Cmr
|
This study |
2221ΔcovR
|
ΔcovR strain |
MGAS2221 covR::kana
|
[16]
|
2221ΔcovRΔccpA
|
ΔcovRΔccpA strain |
2221ΔcovR ccpA::spc
|
This study |
MGAS5005 |
Invasive clinical isolate, serotype M1 |
ΔcovS nt80 |
[67]
|
5005ΔccpA
|
ΔcovSΔccpA strain |
MGAS5005 ccpA::spc
|
[30]
|
comp5005ΔccpA
|
ΔcovSΔccpA strain with CcpA complementation vector |
ΔccpA/ccpA+, Spcr Cmr
|
[30]
|
BL21-CcpA |
E. coli BL21 producing recombinant GAS CcpA |
BL21 pET-His2-CcpA Ampr
|
[30]
|
BL21-CovR |
E. coli BL21 producing recombinant GAS CovR |
BL21 pTXB1-CovR Ampr
|
This study |
BL21-HPr |
E. coli BL21 producing recombinant GAS HPr |
BL21 pET-His2-HPr Ampr
|
This study |
BL21-HPrK/P |
E. coli BL21 producing recombinant GAS HPr kinase/phosphorylase |
BL 21 pET21a-HPrK/P Ampr
|
This study |
pSL60-1 |
Vector containing aad9 gene |
Spcr
|
[68]
|
pDC123 |
Chloramphenicol resistance vector |
Cmr
|
[63]
|
pDCccpA |
pDC123 with CcpA |
Cmr
|
[30]
|
pET-His2-CcpA |
pET-His2 plasmid with GAS CcpA gene |
ccpA+ Ampr
|
[30]
|
pTXB1-CovR |
pTXB1 plasmid with GAS CovR gene |
covR+ Ampr
|
This study |
pET-His2-HPr |
pET-His2 plasmid with GAS HPr gene |
hpr+ Ampr
|
This study |
pET21a-HPrK/P |
pET21a plasmid with GAS HPrK/P gene |
hprK/P+ Ampr
|
This study |