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. 2010 Mar 19;6(3):e1000817. doi: 10.1371/journal.ppat.1000817

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid Description Genotype Reference
MGAS2221 Pharyngitis clinical isolate, serotype M1 Wild-type for covRS [26]
2221ΔccpA ΔccpA strain MGAS2221 ccpA::spc This study
comp2221ΔccpA ΔccpA strain with CcpA complementation vector ΔccpA/ccpA+, Spcr Cmr This study
2221ΔcovR ΔcovR strain MGAS2221 covR::kana [16]
2221ΔcovRΔccpA ΔcovRΔccpA strain 2221ΔcovR ccpA::spc This study
MGAS5005 Invasive clinical isolate, serotype M1 ΔcovS nt80 [67]
5005ΔccpA ΔcovSΔccpA strain MGAS5005 ccpA::spc [30]
comp5005ΔccpA ΔcovSΔccpA strain with CcpA complementation vector ΔccpA/ccpA+, Spcr Cmr [30]
BL21-CcpA E. coli BL21 producing recombinant GAS CcpA BL21 pET-His2-CcpA Ampr [30]
BL21-CovR E. coli BL21 producing recombinant GAS CovR BL21 pTXB1-CovR Ampr This study
BL21-HPr E. coli BL21 producing recombinant GAS HPr BL21 pET-His2-HPr Ampr This study
BL21-HPrK/P E. coli BL21 producing recombinant GAS HPr kinase/phosphorylase BL 21 pET21a-HPrK/P Ampr This study
pSL60-1 Vector containing aad9 gene Spcr [68]
pDC123 Chloramphenicol resistance vector Cmr [63]
pDCccpA pDC123 with CcpA Cmr [30]
pET-His2-CcpA pET-His2 plasmid with GAS CcpA gene ccpA+ Ampr [30]
pTXB1-CovR pTXB1 plasmid with GAS CovR gene covR+ Ampr This study
pET-His2-HPr pET-His2 plasmid with GAS HPr gene hpr+ Ampr This study
pET21a-HPrK/P pET21a plasmid with GAS HPrK/P gene hprK/P+ Ampr This study