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. 2010 Mar 19;6(3):e1000815. doi: 10.1371/journal.ppat.1000815

Figure 3. Effector molecule expression among tgd057-specific CTLs.

Figure 3

Expression of IFN-γ and granzyme B was analyzed in CD8+ T cells from naïve and day 8 CPS-primed mice. A) The frequencies of tgd057-specific CTLs (CD44hi, Kb/SVLAFRRL+, CD8α+, and TCRβ+ cells) was compared between naïve and primed samples ex vivo, upper row. IFN-γ and granzyme B expression was evaluated between CD8+ T cells from the naïve spleen, and total CD8+ T cells and tgd057-specific (Tetramer+) CD8+ T cells from day 8 CPS-primed PECs and splenocytes, lower row. For each of these populations, IFN-γ and granzyme B expression was analyzed after CPS restimulation (B) and peptide restimulation (C). B) Samples were either unstimulated (upper row) or restimulated (lower row) with CPS parasites. C) Samples were directly incubated with either non-cognate (SIINFEKL, upper row) or cognate (SVLAFRRL, lower row) peptide. FACS plots represent one of three mice. One of three independent experiments is shown.