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. 2010 Mar 19;6(3):e1000819. doi: 10.1371/journal.ppat.1000819

Figure 7. CagA translocation is functional in O antigen negative H. pylori strains.

Figure 7

Human gastric epithelial AGS cells were infected with H. pylori G27 (1) wild type, (2) wecA mutant, (3) wzk mutant, or (4) waaL mutant strains. (5) Non-infected AGS cells served as negative control. AGS membranes were examined for translocation and tyrosine phosphorylation of CagA by Western blotting using (A) anti-CagA antibody and (B) anti-phosphotyrosine (pY) antibody. (C) The host membrane marker h-Met was detected with an anti-h-Met antibody for a loading control. Arrows indicate full length phosphorylated CagA. A protein marker standard was included for reference.