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. 2010 Mar 19;5(3):e9647. doi: 10.1371/journal.pone.0009647

Figure 2. RNAi knockdown of Tiam1 expression inhibits neuronal differentiation of PC12 cells in response to NGF.

Figure 2

A) Photomicrographs show PC12 cells transfected with control or pSuper-Tiam1 RNAi (Tiam1 RNAi) together with a GFP expression vector. After 72 h of NGF treatment, the cells were fixed and stained with phalloidin. Arrowheads indicate neuronal cell bodies and arrows denote neurite tips. Scale bar: 10 µm. B) The histogram shows the quantification of the relative number of GFP positive PC12 cells bearing neurites longer than 1.5 cell body diameters after 72 h of treatment with NGF. The results are presented as average ± SD of a representative experiment performed in triplicate. *p<0.001 (ANOVA followed by Student Newman Keuls). The experiment was repeated three times with similar results. C) Endogenous levels of protein were analyzed by immunoblot (IB) in PC12 cells transfected with control or pSuper-Tiam1 RNAi.