Table 1. TCR β-chain VDJ amino acid sequences expanded in response to immunization with peptide 24112.

Individual	TCRVβ Family	Vβa sequence	nDnb	Jβc sequence	TCRJβ Family	HLA-DRβ1
		92		111
Human Influenza	Vβ3S1	LESASTNQTSMYLCASS	STGLP	YGYTFGSGTRLTVVEDLNKVFPPEVAVF	1.2	0401
Antibody producer and protected individuals
Ao191•	Vβ12-3	IQPSEPGDSAVYFCASS	FLEGG	YDYTFGSGTRLTVVDDLSKVFPPTVAVF	1.2	0403
Ao250	Vβ12-1	IQPSEPRDSALYLCASS	QQG	NYDYTFGSGTRLTVVDDLSKVFPPTVAVF	1.2	0403
Ao259	Vβ6-10	LEAAAPSQTSVYFCASS	DLLSA	NYDYTFGSGTRLTVVDDLSKVFPPTVAVF	1.2	0403
Ao149	Vβ12-6	IQPSEPRDSAVYFCASS	LASGS	TDPLYFGPGTRLTVLDDLNKVFPPTVTVA	2.3	0403
Antibody producers and non-protected individuals
Ao239	Vβ5-4	LSSLELGDSALYFCASS	VGIRD	YDYTFGSGTRLTVVDDLSKVFPPTVAVF	1.2	0403
Ao277	Vβ19-12	LTSAQWNQTAFYLCASS	TPI−−	NYDYTFGSGTRLTVVDDLSKVFPPTVAVF	1.2	0403
Non-antibody producers or protected individuals
Ao148•	Vβ15-5	IRSSGLGDAATYLCASS	RDEED	YDYTFGSGTRLTVVDDLSKVFPPTVAVF	1.2	0422
Ao148	Vβ28-9	STNQTSVYLCASS	LYLRG	NYDYTFGSGTRLTVVDDLSKVFPPTVAVF	1.2	0422
Ao224	Vβ28-9	STNQTSLYLCAS	FAGDI	EAFFGEGTKLTVVDDLSKVFPPTVAVF	1.1	0422
Ao142	Vβ9-9	VSGLELGDSALYLCAS	NLVLWTGVR	NYDYTFGSGTRLTVVDDLSKVFPPTVAVF	1.2	0422
Ao148	Vβ7-6	IQRTKQGDSAMYLCASS	PSGAGS	TDPLYFGPGTRLTVLDDLNKVFPPTVAVF	2.3	0422
Ao224	Vβ7-1	IQRTKQGDSAMYLCASS	SDGLY	TDPLYFGPGTRLTVLDDLNKVFPPTVAVF	2.3	0422
Ao142	Vβ5-3	VSGLELGDSALYLCAS	NTGLADCPC	TDPLYFGPGTRLTVLDDLNKVFPPTVAVF	2.3	0422

CDR3 sequences are limited by ⁹²CASS and FGXG¹¹¹ motifs (in bold and italic).

^aVβ: β-chain variable region amino acid sequences.

^bnDn: non-templated diversity-n region.

^cJβ: β-chain diversity joint region families. Big black dots correspond to the Aotus Vβ amino acid sequences used to replace the corresponding residues in the V3S sequence of the HLA-DRβ1*0401–HA–HA 1.7 TCR complex, numbered according to the crystallographic structure reported by Hennecke [20] and shown in the first row.