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. Author manuscript; available in PMC: 2010 Mar 19.
Published in final edited form as: Cell Death Differ. 2009 Jun 19;16(10):1344–1351. doi: 10.1038/cdd.2009.75

Figure 4. DCC-5Fbn triggers cell death via disruption of netrin-1-mediated inhibition of DCC/UNC5H2 pro-apoptotic activity.

Figure 4

(A) HEK293T cells were transiently transfected with a mock (Cont.) or a full-length DCC construct (DCC) and incubated with or without netrin-1 (net) (150 ng/ml) and/or DCC- 5Fbn (800 ng/ml) or the complete extracellular domain of DCC (DCC-EC) (200 ng/ml). Cell death was assessed by trypan blue staining. Inset: DCC immunoblot showing DCC expression level in the different tested conditions. (B) HEK293T cells were transiently transfected with a mock (Cont.) or a full length UNC5H2 construct (UNC5H2) and incubated with or without netrin-1 (net) (500 ng/ml) and/or DCC-5Fbn (1000 ng/ml) or the complete extracellular domain of DCC (DCC-EC) (200 ng/ml). Cell death was measured by fluorescent TUNEL staining. Hoechst staining shows cell nuclei. For each condition, at least 5 randomly chosen fields were analyzed and photographed under epifluorescence microscopy. A representative field is presented from 3 independent experiments. Superimposed photographs (Merge) are shown. Scale bar represents 100 μM.