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. Author manuscript; available in PMC: 2010 Mar 19.
Published in final edited form as: Cell Death Differ. 2009 Jun 19;16(10):1344–1351. doi: 10.1038/cdd.2009.75

Figure 5. DCC-5Fbn triggers HCT116 cell death in vitro and inhibits HCT116 cell metastasis in a chicken model.

Figure 5

(A) Quantification of netrin-1 and netrin-1 receptors. Ratio of netrin-1 and netrin-1 receptors expression to HPRT housekeeping gene is presented. (B) Representative netrin-1 immunohistochemistry on HCT116 tumor cell line. Inset: Control without primary antibody is presented. (C) Quantitative analysis of cell death in HCT116 cell line treated with DCC-5Fbn, with or without addition of netrin-1 in excess to reverse the effect of DCC-5Fbn. Cell death was quantified by trypan blue exclusion assay (below panel) while apoptosis was monitored by measuring relative caspase-3 activity (upper panel). (D) HCT116 cells were grafted in chick chorioallantoic membrane at day 10 and DCC- 5Fbn or PBS was injected on day 10 and day 13. Tumours and lungs were harvested on day 17. Percentage of embryos with lungs invaded by human HCT116 cells after two injections (day 10 and day 13) of either DCC-5Fbn or PBS. * indicates a p<0,05 calculated using a χ-squared test.