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SrtF is required for the efficient cell wall anchoring of the SpaH-type pili. Wild-type C. diphtheriae (A) and its isogenic derivatives ΔsrtA-C, F (designated as srtD+srtE+) (B), or ΔsrtF (C) were immobilized on carbon grids, stained with specific antiserum against SpaH and goat anti-rabbit IgG conjugated to 18 nm gold particles. Samples were viewed by transmission electron microscopy. Scale bars indicate the length of 0.2 μm.
