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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cancer Gene Ther. 2009 Oct 2;17(4):244–255. doi: 10.1038/cgt.2009.70

Figure 3.

Figure 3

VEGF enhances tumor vascular leakiness and promotes Sindbis vector targeting. RD-Sindbis/VEGF vector (~107 particles/mL) were mixed with RD-Sindbis/Fluc vector (~107 particles/mL) at 1:1 ratio. A mixture of RD-Sindbis/LacZ and RD-Sindbis/Fluc was used as a control. SCID mice bearing s.c. BHK tumors received four daily i.p. treatments of vector mixture (500 μL) from day 0–3. For imaging vascular leakiness in tumors, AngioSense 750 (0.66 nmol) was i.v. injected once on day 1. (a) AngioSense retention signal on day 3 shows enhancement of vascular permeability in the tumors of mice receiving the mixture containing RD-Sindbis/VEGF vectors. (b) AngioSense retention signals were analyzed in total fluorescent efficiency on day 1 (100 min after probe injection), 2, 3, 4 and 7. (c) Bioluminescent imaging of luciferase activities indicates VEGF promotes vector delivery and transduction. (d) Quantitative presentation of luciferase activities in tumors.