Figure 5. Histological Analysis of Normal and Tumor-Bearing Pancreas.
A. Consecutive sections from normal rat pancreas stained with H&E (left) or unstained and imaged at 700 nm using a NIR fluorescent microscope (right). Rats were either uninjected (top row) or injected IV with 1.5 mg/kg MB and sacrificed at 15 min post-injection (bottom row). NIR fluorescence images have identical exposure times (5 sec) and normalizations. Results are representative of n = 3 independent experiments. I = pancreatic islets; E = exocrine pancreas.
B. Consecutive sections from insulinoma-bearing pancreas of NOD/ShiLt-Tg(RipTAg)1Lt/J mice stained with H&E (left) or unstained and imaged at 700 nm using an NIR fluorescent microscope (right). Mice were either uninjected (top row) or injected IV with 1.5 mg/kg MB and sacrificed at 15 min post-injection (bottom row). NIR fluorescence images have identical exposure times (5 sec) and normalizations. Results are representative of n = 3 independent experiments. Insulinoma is indicated; E = exocrine pancreas.