Histological validation of HaloTag receptor expression in the HaroTagR-SHIN3 tumor.
A. Formalin-fixed paraffin-embedded tumor tissue specimens were imaged on an Olympus BX61 fluorescence microscope with the appropriate filter sets for HaloTagL-Alexa488 (a) or HaloTagL-TMR (b) or HaloTagL-Dy633 (c) or HaloTagL-IR800 (d).
B. Immunohistochemistry of stm-HaloTag 7 fusion protein, which was induced in HaloTagR-SHIN3 cells, in formalin-fixed paraffin-embedded mouse tumor tissue confirms HaloTagR expression in the HaloTagR-SHIN3 tumor.