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. Author manuscript; available in PMC: 2010 Mar 19.
Published in final edited form as: Bioconjug Chem. 2009 Jul;20(7):1367–1374. doi: 10.1021/bc9001344

Figure 6.

Figure 6

Histological validation of HaloTag receptor expression in the HaroTagR-SHIN3 tumor.

A. Formalin-fixed paraffin-embedded tumor tissue specimens were imaged on an Olympus BX61 fluorescence microscope with the appropriate filter sets for HaloTagL-Alexa488 (a) or HaloTagL-TMR (b) or HaloTagL-Dy633 (c) or HaloTagL-IR800 (d).

B. Immunohistochemistry of stm-HaloTag 7 fusion protein, which was induced in HaloTagR-SHIN3 cells, in formalin-fixed paraffin-embedded mouse tumor tissue confirms HaloTagR expression in the HaloTagR-SHIN3 tumor.