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. Author manuscript; available in PMC: 2010 Mar 19.
Published in final edited form as: Cancer Res. 2009 Aug 25;69(17):6782–6789. doi: 10.1158/0008-5472.CAN-09-0128

Figure 4.

Figure 4

G0S2 promotes apoptosis by preventing Bcl-2/Bax heterodimerization. A, HEK293 cells were co-transfected with plasmids expressing G0S2-HA, FLAG-Bcl-2 and/or V5-Bax. Bcl-2 was immunoprecipitated with an α-FLAG antibody and the immunoprecipitate analyzed with an α-V5 antibody. B, (Left) HeLa cells stably expressing Bcl-2 or vector (Neo) were treated with UV. (Right, top) HeLa/Bcl-2 and HeLa/Neo cells were infected with Ad-LacZ or Ad-G0S2, and monitored for apoptosis 48 hours later. (Right, bottom) Immunoblot analysis monitoring expression of Bcl-2, G0S2 and tubulin. C, H1299 cells were co-transfected with plasmids expressing luciferase and Bcl-2, Bax and/or G0S2. Luciferase activity was quantified and expressed relative to the levels observed in untransfected cells. Error bars represent SD.