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. 1986 May;29(5):930–932. doi: 10.1128/aac.29.5.930

Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization.

N Dickgiesser, B N Kreiswirth
PMCID: PMC284184  PMID: 3729351

Abstract

A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

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Selected References

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