Table 4.
Interaction between plasmid MSH2 alleles and an msh6Δ mutation
| MSH2 | RDKY2706 msh2Δ |
RDKY4136 msh2Δmsh6Δ |
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| Mutation rate |
Mutation rate |
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| Plasmid no. | Allele | Thr+ | Canr | Thr+ | Canr |
| pMSH2 | MSH2 | 21.27 × 10−8 (1) | 2.81 × 10−7 (1) | 4.54 × 10−8 (1) | 4.29 × 10−6 (1) |
| pRS413 | Vector | 1.45 × 10−5 (1142) | 6.49 × 10−6 (23) | 1.19 × 10−5 (262) | 9.37 × 10−6 (2.2) |
| pAG80 | msh2-D164H | 1.24 × 10−7 (9.8) | 7.92 × 10−7 (2.8) | 7.87 × 10−7 (13) | 3.48 × 10−6 (0.8) |
| pAG25 | msh2-G122S | 1.11 × 10−7 (8.7) | 4.65 × 10−7 (1.7) | 2.49 × 10−6 (55) | 7.37 × 10−6 (1.7) |
| pRDK1523 | msh2-T33P | 4.25 × 10−7 (33) | 4.01 × 10−6 (14.3) | 2.86 × 10−5 (630) | 2.21 × 10−5 (5.2) |
| pRDK1526 | msh2-L99P | 6.43 × 10−7 (51) | 9.97 × 10−7 (3.5) | 1.70 × 10−5 (374) | 1.13 × 10−5 (2.6) |
| pRDK1527 | msh2-L102H | 1.44 × 10−8 (1.1) | 3.04 × 10−7 (1.1) | 1.15 × 10−5 (253) | 1.09 × 10−5 (2.5) |
| pRDK1528 | msh2-Y109C | 5.70 × 10−8 (4.5) | 4.04 × 10−7 (1.44) | 2.75 × 10−5 (606) | 2.02 × 10−5 (4.7) |
All of the indicated MSH2 alleles were made by site-directed mutagenesis in the pRS413 HIS3 MSH2 vector pMSH2; two of the plasmid mutants (pAG80 and pAG25) were obtained from Dr. Alison Gammie (Princeton University, Princeton, NJ). The number in () is the fold-increase in mutation rate relative to the value obtained with the pMSH2 wild-type plasmid.