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. 2010 Mar 1;107(11):5100–5105. doi: 10.1073/pnas.0913108107

Fig. 5.

Fig. 5.

KACL on keratinocytes stimulates NKp65-mediated degranulation. (A) Predominance of KACL transcripts in human skin. Relative levels of KACL transcripts in human tissues were determined by real-time PCR. (B) KACL is expressed in the epidermis of human skin. In situ hybridization of human skin with a sense KACL probe (control, Upper) and an antisense KACL probe (Lower). (C) Keratinocytes express surface KACL. Freshly isolated human keratinocytes were stained with OMA1 F(ab)2 or control F(ab)2, or with NKp65 tetramers after preincubation with control F(ab)2 or OMA1 F(ab)2. (D and E) KACL-mediated activation of NK92MI-NKp65 by keratinocytes. Degranulating (CD107a+) NK92MI-NKp65 (NKp44+ cells) after coculture with freshly isolated keratinocytes in presence of OMA1 or isotype control. (D) One representative dot plot analysis and (E) summary of five independent experiments using keratinocytes of different donors.