Fig. 6.

(A) Comparison of plasma glucose levels in type 1 diabetic NOD mice treated with twice daily injection of insulin alone at a total dose of 0.2 U/day (▲), twice daily insulin alone at a total dose of 0.02 U/day fed ad libitum (○), twice daily insulin PF to the leptin-treated group (□), or a total daily dose of insulin of 0.02 U/day plus leptin injected at the doses and times indicated by arrows (•). (B) Evidence that much lower doses of infused leptin without insulin are also effective in reducing the hyperglycemia of T1DM. Blood glucose levels of diabetic NOD mice treated for 20 days with leptin monotherapy by osmotic infusion pump are shown. Priming doses during the first 7 days ranged from 1/500 to 1/2,000 of the doses injected with low-dose insulin, and maintenance doses of infused leptin thereafter ranged from 1/500 to 1/2,500 of the doses of injected leptin employed.