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. 2010 Mar 1;107(11):5047–5052. doi: 10.1073/pnas.1000735107

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Fig. 6. — Multiple MTSs support allotopic expression of COX2^W56R. (A) Wild-type (W) and cox2-60 (M) yeast strains harboring the indicated plasmids were serially diluted and stamped on solid media containing either glucose (YP + glucose) or glycerol (YP + glycerol). Four MTS-COX2^W56R-ADH1³^′-UTR expression plasmids (MTSs OXA1, 2×OXA1, SU9, and 2×SU9) were able to restore glycerol-dependent growth of the cox2-60 mutant. (B) Expression of Cox2^W56R in yeast cells harboring MTS-COX2^W56R-ADH1³^′-UTR expression constructs. Amounts of yeast actin present in samples are shown for comparison. Cox2 species migrating at the position of the mature, mitochondria-coded Cox2 are indicated with an arrow.