Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Endocrinol. 2009 Jul 27;203(1):99–110. doi: 10.1677/JOE-09-0221

Figure 6.

Figure 6

NRL-PRL glands exhibit elevated pStat5, pERK1/2 and pAkt. A, B. Phosphorylated Stat5 (pStat5) was examined in morphologically normal structures of age-matched 6 month old nontransgenic and NRL-PRL intact nonparous females (A), and normal structures and epithelial hyperplasias (EH) in glands of 2 year old NRL-PRL females (B). pStat5-labeled cells were detected and quantitated as described in Materials and methods and expressed as mean ± s.d. Lower case letters denote significant differences using ANOVA followed by Student-Newman-Keuls Multiple Comparison test (p<0.05). C. Glands of NRL-PRL females at 12 weeks of age contain elevated pERK1/2 and pAkt, compared to nontransgenic littermates. Each lane represents mammary homogenate from a single 12 week old NRL-PRL female or nontransgenic littermate (FVB), examined by immunoblotting as shown. D. The ratio of phosphorylated: total kinase for each animal shown in (C) was calculated as described in the methods. An asterisk indicates a significant difference between genotypes by a one-tailed Student's t test (*, p<0.05).