Figure 2. Kinetics of vaccinia-specific antibody response following scarification with vaccinia virus.
K14/IL-1α and WT mice were skin scarified at the base of their tails with 2 × 106 PFU of WR-GFP vaccinia virus. At various time points after immunization (weekly for weeks 1 to 4 and biweekly for weeks 6 to 12), mice from each group were bled and their sera were collected. Sera were also collected from control, uninfected (week 0) mice. The kinetics of the vaccinia-specific antibody response in the sera of individual mice at each time point was measured by ELISA. Data represent means ± SE using 6 mice per group and are representative of four independent experiments. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.