Cells recovered from inguinal lymph nodes harvested at the time points indicated were stained for CD11c and MHC Class II, CD80 or CD86. Bars represent mean fluorescence intensity of MHC Class II, CD80 and CD86 on CD11c+ gated cells. Data represent the means ± SE of four to five individual mice in one experiment. * = p < 0.05, ** = p < 0.01, *** = p < 0.001, n.s. = not significant.