Figure 3. Stat3 ablation leads to decreased VEGF production and angiogenesis.
Decreased VEGF protein levels in Stat3flx/flx/NIC tumors by immunoblotting (A) and ELISA (B) assays (average VEGF quantity ±s.e.m.). (C) Immunohistochemical staining using a CD31 antibody (left panel) and quantification of the average number (±s.e.m.) of CD31 positive pixels (right panel). Scale bar: 100 μm. (p values were calculated using a two-tailed student’s t-test; *p=0.017).