Table 1. Expression of neural stem cell–associated molecules and of transcription factors by CSC and FBS tumor lines.
| Cell line | Molecule* | ||||||
|---|---|---|---|---|---|---|---|
| c-Myc | Nestin | Nanog | S100A4 | S100A6 | Sall4 | Sox2 | |
| 0627 CSC | 2 (70) | n.d. | 3 (97) | 3 (98) | 4 (97) | n.d. | 28 (100) |
| 080125 CSC | 2 (79) | n.d. | 2 (82) | 2 (92) | 2 (89) | 5 (100) | 7 (99) |
| 080125 FBS | 2 (88) | n.d. | 4 (99) | 2 (97) | 2 (84) | 8 (99) | 3 (99) |
| 080418 CSC | 2 (67) | 35 (99) | 2 (82) | 2 (92) | 4 (89) | 4 (100) | 38 (100) |
| 080418 FBS | 4 (89) | 15 (99) | 2 (99) | 1 (6) | 2 (43) | 3 (100) | 20 (100) |
| 080325 CSC | 1 (42) | n.d. | 3 (95) | 4 (97) | 2 (34) | 3 (99) | 15 (99) |
| 080325 FBS | 1 (3) | n.d. | 4 (99) | 1 (2) | 1 (1) | n.d. | 8 (79) |
| 071011 CSC | 1 (7) | 108 (99) | 2 (92) | 2 (61) | 3 (71) | n.d. | n.d. |
| 071011 FBS | 1 (1) | 5 (97) | 2 (85) | 2 (77) | 2 (57) | n.d. | n.d. |
| 070104 CSC | 2 (94) | 253 (75) | 4 (96) | 6 (97) | 5 (96) | 7 (78) | 35 (100) |
| EBV-B 1869 | 1 (2) | 1 (4) | 1 (6) | 1 (6) | 1 (3) | n.d. | 5 (98) |
Abbreviation: n.d., not done.
Note: Data are represented as MRFI that is the ratio between the mean of fluorescence intensity of cells stained with the selected mAb and that of the negative control; bold values indicate significant MRFI (≥ 2). The numbers in parenthesis represent the percentage of positive cells. The experiment has been repeated thrice; SD for each value ≥ 1 ≤ 4.
*
The expression of the indicated molecules was evaluated by intracellular staining with specific mAbs and cytofluorimetric analysis; see Materials and Methods.