Table 2. Expression of MHC class I and class II, and NKG2DLs molecules and their modulation by IFN-γ by GBM CSCs and FBS tumor cell lines.
| Cell line | IFN-γ* | Molecule† | |||||||
|---|---|---|---|---|---|---|---|---|---|
| MHC I | MHC II | MICA | MICB | ULBP-1 | ULBP-2 | ULBP-3 | ULBP-4 | ||
| 0627 CSCs | − | 7 (94) | 6 (31) | 4 (41) | 1 (6) | 1 (1) | 1 (5) | 1 (1) | 1 (2) |
| + | 8 (100) | 7 (52) | 4 (55) | 1 (5) | 1 (1) | 1 (7) | 1 (1) | 1 (1) | |
| 0627 FBS | − | 4 (81) | 4 (51) | 1 (2) | 1 (1) | 1 (2) | 1 (1) | 1 (1) | 3 (32) |
| + | 8 (100) | 4 (80) | 1 (3) | 1 (1) | 1 (3) | 1 (1) | 1 (1) | 3 (45) | |
| 080125 CSCs | − | 2 (97) | 1 (2) | 1 (4) | 1 (2) | 1 (1) | 1 (4) | 1 (3) | 2 (24) |
| + | 14 (100) | 1 (6) | 1 (4) | 1 (2) | 1 (2) | 2 (20) | 2 (25) | 2 (33) | |
| 080125 FBS | − | 3 (94) | 1 (2) | 3 (95) | 1 (2) | 1 (1) | 2 (14) | 1 (3) | 2 (45) |
| + | 6 (100) | 1 (8) | 3 (98) | 1 (3) | 1 (3) | 2 (14) | 1 (4) | 2 (47) | |
| 080418 CSCs | − | 1 (8) | 1 (2) | 1 (4) | 1 (1) | 1 (2) | 2 (10) | 3 (20) | 3 (40) |
| + | 3 (95) | 1 (6) | 1 (4) | 1 (2) | 1 (2) | 2 (26) | 3 (31) | 3 (55) | |
| 080418 FBS | − | 3 (96) | 1 (3) | 3 (95) | 1 (2) | 1 (3) | 1 (4) | 3 (15) | 1 (4) |
| + | 15 (96) | 1 (8) | 3 (98) | 1 (2) | 1 (3) | 1 (3) | 3 (45) | 1 (6) | |
| 080201 CSCs | − | 2 (65) | 1 (2) | 2 (27) | 2 (20) | 2 (15) | 2 (12) | 1 (3) | 2 (18) |
| + | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
| 080201 FBS | − | 4 (99) | 1 (1) | 1 (6) | 1 (4) | 1 (4) | 2 (22) | 1 (3) | 2 (18) |
| + | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
| 071011 CSCs | − | 6 (98) | 1 (1) | 1 (5) | 1 (4) | 1 (5) | 2 (2) | 1 (4) | 2 (21) |
| + | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
| 071011 FBS | − | 26 (99) | 2 (76) | 2 (96) | 2 (93) | 2 (94) | 3 (95) | 4 (96) | 4 (98) |
| + | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
| 080325 CSCs | − | 30 (99) | 1 (4) | 1 (5) | 2 (67) | 1 (3) | 2 (81) | 3 (80) | 2 (77) |
| + | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | |
| 070104 CSCs | − | 3 (20) | 2 (18) | 1 (2) | 1 (2) | 1 (3) | 1 (2) | 1 (2) | 1 (2) |
| + | 12 (98) | 4 (67) | 1 (3) | 1 (4) | 1 (4) | 1 (3) | 1 (2) | 1 (2) | |
Abbreviation: n.d., not done.
Note: Values are indicated as MRFI that is the ratio between the mean of fluorescence intensity of cells stained with the selected mAb and that of the negative control; bold values indicate significant MRFI (≥ 2). The numbers in parenthesis represent the percentage of positive cells. The experiment has been repeated thrice; SD for each value ≥ 1.2 ≤ 4.
*
Cells were treated or not (±) for 48 h with 500 IU/mL of IFN-γ
†
Expression of MHC molecules and NKG2DLs was evaluated by immunofluorescence and cytofluorimetric analysis.