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. 2010 Feb 22;107(10):4567–4572. doi: 10.1073/pnas.0912305107

Fig. 2.

Fig. 2.

Correlation of 16S rRNA processing and translational fidelity in a strain lacking r-protein S15. (A) Primer extension analysis of the 5′ end of 16S rRNA isolated from sucrose gradient purified 30S subunits (lanes 1 and 2) and 70S ribosomes (lanes 3 and 4) from either the parental W3110 (lanes 1 and 3) or ΔrpsO strain (lanes 2 and 4). (B) Effect of deletion of rpsO on frameshifting errors and stop-codon readthrough in vivo. The ratio of units of β-galactosidase activity (obtained with the mutant lacZ reporters) to the β-galactosidase units (obtained with WT lacZ) in ΔlacZ ΔrpsO strain is normalized to that in an isogenic lacZ deficient MC252 strain. Error bars represent SD.