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. 2010 Feb 22;107(10):4567–4572. doi: 10.1073/pnas.0912305107

Fig. 3.

Fig. 3.

Effects of deletion of RNase G on 16S rRNA maturation, translational fidelity, and aminoglycoside susceptibility. (A) Primer extension analysis of the 5′ end of 16S rRNA isolated from sucrose gradient purified 30S subunits (lanes 1 and 2) and 70S ribosomes (lanes 3 and 4) from either the WT BW25113 (lanes 1 and 3) or Δrng strain (lanes 2 and 4). The mature 5′ end and the precursor forms (lp16S and sp16S rRNA) are indicated. (B) Effect of deletion of rng on frameshifting errors and stop-codon readthrough in vivo monitored by β-galactosidase assay in Δrng and parental BW25113 strains as described in Fig. 2 B. (C) Dilution plating (10-fold serial dilutions) of MG1655 (parent of Δrng) and Δrng strains on LB plates as indicated in presence of neomycin (1 ug/mL). (D) Same as C in presence of paromomycin (1 ug/mL).